Composition principle of the upgraded spectrophoto

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Hengaode instrument upgraded spectrophotometer instrument composition principle

spectrophotometer, also known as spectrometer, avoids the subsequent loss caused by unqualified parts. It is a scientific instrument that decomposes complex light into spectral lines. The measurement range generally includes the visible light region with a wavelength range of 380~780

nm and the ultraviolet light region with a wavelength range of 200~380

nm. Different light sources have their own emission spectra, so different luminaries can be used as the light source of the instrument. Emission spectrum of tungsten lamp: after the spectral light of 380~780nm wavelength emitted by tungsten lamp light source is refracted through the prism, a continuous chromatography composed of red, orange, yellow, green, blue, indigo and violet can be obtained; The chromatography can be used as the light source of visible spectrophotometer

photometric definition

spectrophotometry is the absorbance of light at a specific wavelength or within a certain wavelength range, and carries out qualitative or quantitative analysis of the substance. The commonly used wavelength range is: (1) the ultraviolet light region of 200~380nm, (2) the visible light region of 380~780nm, (3) 2.5~25 μ M (4000cm; ~400cm in wave number) Infrared light area. The instruments used are ultraviolet and visible spectrophotometer (or), or. In order to ensure the precision and accuracy of measurement, all instruments should be calibrated regularly in accordance with the national metrological verification regulations or the provisions of this appendix

instrument composition

spectrophotometer has become a conventional instrument in modern molecular biology laboratory. It is often used to quantify nucleic acid, protein and bacterial growth concentration

the instrument is mainly composed of light source, monochromator, sample room, detector, signal processor and display and storage system


the spectrophotometer uses a light source that can produce multiple wavelengths. Through a series of spectroscopic devices, a light source with a specific wavelength is generated. After the light passes through the tested sample, part of the light is absorbed, and the absorption value of the sample is calculated, which is converted into the concentration of the sample. The absorbance value of the sample is directly proportional to the concentration of the sample

when monochromatic light radiation passes through the solution of the substance to be measured, the amount absorbed by the substance is directly proportional to the concentration of the substance and the thickness of the liquid layer (optical path length), and the relationship is as follows:

a=-lg (i/i)=- Lgt=klc


is the absorbance

i。 Is the intensity of incident monochromatic light


is the intensity of transmitted monochromatic light


is the transmittance of the substance


is the molar absorption coefficient


is the optical path of the analyzed substance, that is, the side length of the cuvette

instrument features

unique dual optical path and dual beam optical system, higher resolution, lower stray light, stronger stability and reliability, More accurate analysis

use 320*240 point array to highlight 6

"LCD, with clear display and complete information

the unique long optical path design makes the resolution of the instrument higher, especially suitable for micro testing

the powerful data processing function enables the test results to be fully applied, and the user is simpler and faster

the suspension optical system design is adopted, and the overall optical path is independently fixed on a 16mm thick aluminum deformable base. The deformation of the base plate and the external vibration are not harmful to the optical system Have any impact, thus greatly improving the stability and reliability of the instrument

adopt synchronous sine mechanism, with high wavelength accuracy and good repeatability

adopt arm system

0.1/0.2/0.5/1.0/2.0/4.0 six gear spectral bandwidth can be automatically selected to meet the measurement needs of different users

24 bit high-speed, high-precision a/d rotation. Manually control the loading speed valve to control the rising speed of the piston (the speed is 1-BODY with the safety valve), The instrument has higher accuracy and faster reaction speed

the main components are imported, which makes the instrument less stray light, more stable and reliable

the function is more powerful. The host can independently complete photometric measurement, quantitative measurement, spectral scanning, dynamics, DNA/protein testing, multi wavelength testing and data printing

operation method

1 Turn on the power, turn on the instrument switch, lift the dark box cover of the sample room, and preheat for 10 minutes

2. Adjust the sensitivity switch to "1" (if the zero point adjuster cannot be adjusted to "0", the higher gear shall be selected.)

3. Turn the wavelength selection button according to the desired wavelength

4. Pour the blank solution and the measuring solution into 3/4 of the cuvette respectively, wipe the outer wall with the lens wiping paper, put it into the sample room, and align the blank tube with the light path

5. Adjust the zero point adjuster when the black box cover is open, so that the pointer of the reading disc points to t=0

6. Cover the black box, adjust the "100" regulator so that the t=100 of the blank tube. After the pointer is stable, gradually pull out the sample slide rod, read out the optical density value of the measuring tube respectively, and record it

7. After colorimetry, turn off the power, take out the cuvette and wash it, and wipe the sample room with a soft cloth or paper


1 The instrument should be placed in a dry room and placed on a firm and stable workbench when in use. The indoor lighting should not be too strong. In hot weather, you can't use an electric fan to blow directly to the instrument to prevent the bulb filament from being bright and unstable

2. Before using this instrument, the user should first understand the structure and working principle of this instrument, as well as the functions of each control knob. Before turning on the power supply, the safety performance of the instrument should be checked. The power connection should be firm and the power supply should be good. The starting position of each adjusting knob should be correct, and then press the power switch

3. When the instrument is not powered on, the meter pointer must be on the "0" mark. If this is not the case, it can be adjusted with the correction screw on the meter

routine maintenance

analytical instrument workers should understand the routine maintenance of the instrument and the simple test methods for the main technical indicators, and often maintain and test the instrument to ensure that the instrument works in the best state

first, temperature and humidity are important factors affecting the performance of the instrument. They can cause the corrosion of mechanical parts, reduce the smoothness of the metal mirror, and cause the error or performance degradation of the mechanical part of the instrument; The aluminum film of optical components such as grating, reflector, focusing lens, etc. is corroded, resulting in insufficient light energy, stray light, noise, etc., and even the instrument stops working, thus affecting the service life of the instrument. It should be corrected regularly during maintenance. There should be an instrument room with constant humidity in four seasons, equipped with constant temperature equipment, especially the laboratory located in the south

second, the dust and corrosive gases in the environment can also affect smart phones, ultrabooks, LEDs, smart appliances and other products. They are the highlight of the electronic information and electrical industry. The flexibility of mechanical systems, reducing the reliability of various limit switches, keys, photoelectric couplers, is also one of the reasons for the corrosion of aluminum film of essential components. Therefore, it must be cleaned regularly to ensure the sanitary conditions of the environment and the instrument room and prevent dust

III. after a certain period of use, a certain amount of dust will accumulate inside the instrument. It is best for the maintenance engineer or under the guidance of the engineer to regularly open the outer cover of the instrument to remove dust inside. At the same time, retighten the radiator of each heating element, clean the sealing window of the optical box, calibrate the optical path if necessary, clean and lubricate the mechanical part, and finally restore the original state, Then carry out some necessary detection, adjustment and recording

maintenance of folding section

spectrophotometer is a kind of precision instrument. Due to various reasons such as working environment and operation method, its technical condition will inevitably change, which may affect the performance of the equipment, and even induce equipment failure and accidents. Therefore, analysts must understand the basic principle and instructions of spectrophotometer, find and eliminate these hidden dangers in time, and repair the faults in time to ensure the normal operation of instruments and equipment

1) if the test wavelength is changed significantly, it is necessary to wait a moment, and then re calibrate the "0" and "100%" points after the lamp is heated and balanced. Then measure

2) when the pointer instrument is not powered on, the pointer of the meter must be on the zero scale. If this is not the case, mechanical zeroing is required

3) after the use of the cuvette, please wash it immediately with distilled water and wipe away the traces of water with clean and soft gauze to prevent the surface finish from being damaged and affect the light transmittance of the cuvette

4) operators should not easily move bulbs and reflector lamps to avoid affecting light efficiency

5) 1900 isospectrophotometer, because its photoelectric receiving device is a photomultiplier tube, which is characterized by large amplification, can be used to detect weak photoelectric signals, but not strong light. Otherwise, it is easy to produce signal drift and reduce sensitivity. In view of the above characteristics, when repairing and using such instruments, pay attention not to expose the photomultiplier tube to light for a long time. Therefore, when preheating, open the cover of the cuvette or use the light barrier bar to avoid its performance drift caused by long-term irradiation and unstable operation

6) after the sensitivity of the amplifier is shifted, it must be reset

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